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PROTOCOLS
Protocol ID  (downloads) Laboratory Type Description
clone1 Dumont cloning cloning into 40 vector
exp1 Dumont expression Gal expression in YPD
exp2 Dumont expression ADH2 expression in YPG
pTAL Dumont purification protein purification on Talon
pTAL-SEC Dumont purification protein purification on Talon and SEC
pTAL-IgG-SEC Dumont purification protein purification on Talon, IgG, and SEC
pIgG-SEC Dumont purification protein purification on IgG and SEC
cxcl1 Phizicky cloning PCR and ligase independent cloning
cxex1 Phizicky expression transformation into yeast, 40 mL growth, IgG cleavage
cxpur1 Phizicky purification 900 mL growth and IgG purification
cxpur2 Phizicky purification 900 mL growth and IgG -Talon purification
cxpur3 Phizicky purification 900 mL growth and Talon - IgG purification