| Protocol ID (downloads) |
Laboratory |
Type |
Description |
| clone1 |
Dumont |
cloning |
cloning into 40 vector |
| exp1 |
Dumont |
expression |
Gal expression in YPD |
| exp2 |
Dumont |
expression |
ADH2 expression in YPG |
| pTAL |
Dumont |
purification |
protein purification on Talon |
| pTAL-SEC |
Dumont |
purification |
protein purification on Talon and SEC |
| pTAL-IgG-SEC |
Dumont |
purification |
protein purification on Talon, IgG, and SEC |
| pIgG-SEC |
Dumont |
purification |
protein purification on IgG and SEC |
| cxcl1 |
Phizicky |
cloning |
PCR and ligase independent cloning |
| cxex1 |
Phizicky |
expression |
transformation into yeast, 40 mL growth, IgG cleavage |
| cxpur1 |
Phizicky |
purification |
900 mL growth and IgG purification |
| cxpur2 |
Phizicky |
purification |
900 mL growth and IgG -Talon purification |
| cxpur3 |
Phizicky |
purification |
900 mL growth and Talon - IgG purification |
